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Inject the needle into the syringe chain, then remove the filter head (0.45, 0.25um) from the needle sleeve and discard the 4, 5 drops of the initial filtrate to remove the filtrate;
Micro-injectors can also be put on, are the injection heads not all uniform? If it is not possible to seal with a sealing film.
This is the end of the introduction of How to filter the LC sample. I hope it can help you.