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definition
A guard column, in a high performance liquid chromatograph(2ml Agilent wide opening vials), is installed between the injector and the analytical column, through the mobile phase and the sample solution.
effect
Pre-capture can protect the column and extend the life of the analytical column by being firmly adsorbed by the analytical column and not being washed by the mobile phase.
other
1: filler
The packing of the guard column is generally the same as the analytical column.
2: length
The length of the guard column is generally only 1-2 cm.
3: Cleaning
The guard column should first be removed from the analytical column during cleaning to prevent the eluent from entering the analytical column; then select the appropriate mobile phase for flushing.
This is the end of the introduction of What is the role of the chromatographic guard column?. I hope it can help you.
