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Why is it important to use Agilent-certified vials on my Agilent autosampler?
Agilent Certified Vials are the only vials in the industry designed and tested to be fully compatible with our autosampler. Agilent designs these Agilent-certified vials with the same attention and precision as their instruments, columns, and part designs. Many Agilent autosamplers use robotic arms to grab sample bottlenecks. Therefore, the correct height of the sample neck and shoulder is critical to prevent the vial from falling and sample loss. Only Agilent-certified vials are available for Agilent autosamplers, and competitors' products do not meet our exact specifications, resulting in instrument downtime and potentially valuable sample loss.
Are Agilent-certified caps and end cannulas as important?
In order to avoid leakage and sample loss due to evaporation, the inner cannula must be placed in the vial - and the entire device must be completely sealed when capping. To prevent needle damage and ensure proper sample extraction when using the inner cannula, you should also consider the sampling depth of the autosampler needle.
The thread on the Agilent-certified threaded vial is designed to match the Agilent-certified cap. The use of non-Agilent-certified caps on these vials does not guarantee a safe seal and can result in sample loss. In addition, certified jaw vials and caps are tested with more than 100,000 Agilent autosamplers to confirm perfect fit and safety seals.
Do I need to pay attention to the compatibility between the sample and the vial?
For highly sensitive samples such as pesticides or semi-volatiles and samples with a sudden change in pH, deactivating vials is a good choice because their surfaces are more hydrophobic and inert. For more stringent, demanding applications such as mass spectrometry, it is recommended to use deactivated vials to prevent sample interaction prior to analysis.
Polypropylene vials are a good choice for bio-applications and applications involving high-metal sample applications such as ion chromatography, AA or ICP-MS. Agilent thoroughly tested and evaluated a range of polypropylene materials prior to selecting the material grade for polypropylene vials. Our polypropylene vials contain virtually no leachable impurities to ensure sample integrity.
Can I rely on consistency between vials?
The autosampler vial may vary in size and wall weight if tight tolerances are not controlled. This affects the volume of the sample in the vial, especially for small samples. Our strict tolerance control runs through the entire manufacturing process, from the initial design of Zui to the photoelectric scanning inspection process after Zui, ensuring the accuracy of each vial and cap specification.
Agilent-certified vials meet industry-standard tolerance requirements to ensure consistency between batches and batches and between vials and vials.
Why should I buy a vial from Agilent?
Vial integrity, cleanliness and uniformity are critical to today's demanding applications. That's why Agilent vials and caps are designed with the same engineering expertise as Agilent instruments. All Agilent-certified vials are manufactured in an ISO9001 clean environment and must pass a 33-point visual inspection, unique packaging to ensure cleanliness and safety during transportation.
Does my application need to repeat injections or sample storage from the 2 mL clear Agilent vials?
Resealability is an important factor to consider when choosing a septum. PTFE/red rubber septa are not recommended for multiple injections or for storage for later analysis. PTFE/silicone rubber/PTFE is always a good choice when it takes a long time between injections or when adding any type of standard.
How much strength does it take to pierce the septum?
Typically, silicone rubber septa are easier to puncture than red rubber or butyl rubber septa. For thicker, difficult to puncture septa, a small (23 gauge) needle may be required. Or a more powerful S-type needle.
Pre-perforated septum is a good choice for Zui, easy to puncture and reduce the possibility of debris.
All Agilent-certified septa are suitable for use with the Agilent Autosampler and are perfectly matched to the cap.
What temperature range does my application require?
At higher temperatures, the septum will degrade and cause contamination of your sample. The red rubber septum is stable only below 90 °C and is therefore not suitable for high temperature applications. Typically, PTFE-lined silicone rubber septa are a good choice for a variety of temperatures.
Newer headspace applications require temperatures up to 300 °C, and Agilent's new high-performance septa are made of special patented materials to ensure small degradation at extreme temperatures.
How can I avoid the common problems caused by the septum?
Debris
Debris is generated when the diameter of the needle is too large relative to the septum or the septum material cannot withstand multiple injections. At this point, the septum material will fall into the vial and contaminate the sample. Here's how to prevent debris:
1 Select a PTFE-lined septum to prevent the septum material from entering the sample
2 Make sure the needle is not damaged. Consider using a side hole needle instead of a tapered needle.
3 Using pre-punched septa almost completely eliminates debris
Creating a vacuum
Sometimes aspirating a sample from 2 mL clear Agilent vials can result in a vacuum. Some simple steps can prevent this from happening:
1 Use a pre-punched septum to help balance the pressure on both sides of the cap
2 don't fill too much
3 Do not exceed 50 μL in one injection (from a 2 mL clear Agilent vials)
This is the end of the introduction of Frequently asked questions about Agilent vials and caps. I hope it can help you.
