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As a separation technology and method, chromatography has been more than 100 years since the beginning of this century, and has now become an important branch of analytical chemistry. With the development of science and technology, the need for production and life has led to the further development of chromatographic technology. In the future, new joint technologies will continue to be applied. The importance of column maintenance and maintenance is summarized as 9 points.
1 column can not collide, bend or strong vibration under any circumstances; 2 when the HPLC column and chromatograph are connected, the valve or pipeline must be cleaned; 3 pay attention to the degassing of the mobile phase; 4 avoid using high viscosity solvent as Mobile phase; 5 injection sample to be purified; 6 strict control of injection volume; 7 after the end of the analysis work, the sample remaining in the injection valve should be cleaned; 8 after the end of the analysis, the appropriate solvent should be used to clean the column ; 9 If the analytical HPLC column is not used for a long time, it should be stored and sealed with a suitable organic solvent.
As humans enter a new era, people face the challenges of rapid development in the fields of information science, life sciences, materials science, and environmental science. The demand for talents in these fields has become a crucial factor for the country's high development. Chromatography is an indispensable tool and tool for life sciences, materials science, and environmental science.
This is the end of the introduction of How much do you know about the column? I hope it can help you.