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The column is a column packed with a stationary phase to separate the mixed components hplc vials manufacturer. Mostly made of metal or glass. It has a shape of a straight tube, a coil, a U-tube, and the like. As a separation technology and method, chromatography has been more than 100 years since the beginning of this century, and has now become an important branch of analytical chemistry. As humans enter a new era, people face the challenges of rapid development in the fields of information science, life sciences, materials science, and environmental science. The demand for talents in these fields has become a crucial factor for the country's high development.
Before using the column, it is best to test the performance of the column hplc vials manufacturer and save the results as a reference for future evaluation of column performance changes. However, it should be noted that the performance of the column may vary depending on the conditions of the sample, mobile phase, and column temperature used. In addition, the column performance test is performed according to the conditions in the column factory report. The results obtained are comparable. After testing the column before use, we need to maintain the column according to different conditions: 1 reversed phase column daily maintenance after the experiment: use buffer or salt mobile phase, apply 10% after the experiment is completed Rinse in methanol/water for 30 minutes, wash off the salt in the column, and rinse with methanol for 30 minutes. Note: The column should not be rinsed with pure water. 10% methanol should be added to the water to prevent the packing from collapsing. 2 Long-term storage of the column: If the column is to be stored for a long time, it must be stored in a suitable solvent. For the reverse phase column, it can be stored in pure methanol or acetonitrile. The normal phase column can be stored in purely dehydrated pure hexane. The ion exchange column can be stored in water and the plug attached to the new column is plugged hplc vials manufacturer. The stored temperature is good at room temperature.
Strengthening the maintenance and maintenance of the column is of great significance for the development of high-pressure liquid chromatography. The high-pressure liquid chromatography system includes the column. Therefore, strengthening the maintenance and maintenance of the column is conducive to the development of high-pressure liquid chromatography and promotes China. The development of the medical profession.
This is the end of the introduction of You should know the column maintenance process. I hope it can help you.
