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Chromatography, also known as chromatography gas chromatograph manufacturer, is a high-performance physical separation technique that is used in analytical chemistry and with appropriate detection methods to become a chromatographic method.
The zui early application of chromatography is for separating plant pigments by placing calcium carbonate in a glass tube and pouring petroleum ether containing plant pigment (plant leaf extract) into a tube. At this point, a mixed band of several colors appears immediately at the upper end of the glass tube. Then rinse with pure petroleum ether. With the addition of petroleum ether, the band will continuously move downwards and gradually divide into bands of several different colors. After further washing, the pigments of various colors can be respectively connected and can be separately separated. Identification. Chromatography is also named after this.
The current chromatography method has long been not limited to the separation of pigments, and its method has been greatly developed, but the principle of separation is still the same. We still call it chromatographic analysis.
From the above method, there are two phases in the chromatography, one phase is stationary, we call it the stationary phase, and the other phase flows through the stationary phase, which we call the mobile phase.
The principle of separation of chromatography is to separate the various substances to be separated in the two phases, such as the partition coefficient, adsorption capacity and the like.
An external force is used to pass the mobile phase (gas, liquid) containing the sample through a surface of the stationary phase that is fixed in the column or on the plate and is incompatible with the flow. As the mixture carried in the mobile phase flows through the stationary phase, the components of the mixture interact with the stationary phase.
Due to the difference in properties and structure of the components in the mixture, the magnitude and strength of the force generated between the stationary phase and the stationary phase are different. As the mobile phase moves, the mixture undergoes repeated distribution and balance between the two phases, The components are retained by the stationary phase for a different period of time, thereby flowing out of the stationary phase in a certain order. In combination with an appropriate post-column detection method, the separation and detection of each component in the mixture is achieved gas chromatograph manufacturer.
This is the end of the introduction of How does a gas chromatograph work. I hope it can help you.
