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In fact, the separation effect of the GC column depends mainly on its stationary phase, column length, column inner diameter, and liquid film thickness. In principle, the separation effect of these columns with the same factors is exactly the same. With this in mind, you now have to choose your GC column based on this more fundamental basis, without having to purchase expensive standard-designated GC columns. Let's take a look at these issues.
1) Selection of column length
The resolution is proportional to the square root of the GC column length. In the case where the other conditions are the same, a column length of 4 times is required to obtain a double resolution. Shorter columns are suitable for simpler samples, especially those consisting of components that differ greatly in structure, polarity and volatility. Note that the increase in column length also increases the analysis time.
2) Selection of column inner diameter
The column diameter directly affects column efficiency, retention characteristics, and sample capacity. Small-bore columns have higher efficiency than large-diameter columns, but the column capacity is smaller.
3) Choice of liquid film thickness
Thick film is advantageous for the analysis of low boiling compounds. The thickness of the liquid film affects the retention characteristics of the GC column and the column capacity. The thickness increases and the retention also increases.
4) Selection of stationary phase
Different fixed and different analytes have different effects. According to the principle of similar compatibility, the closer the properties are, the greater the flow resistance relative to it is, and the longer the retention time is. Columns use this principle to separate mixtures of different properties from each other.
Gas chromatography columns are used in a wide variety of applications. In addition to its quantitative and qualitative analysis, it also measures the physicochemical constants of a sample on a stationary phase.
This is the end of the introduction of What are the choices of GC columns. I hope it can help you.
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