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Commonly used quantitative methods for chromatographic analysis: normalization, internal standard and internal (incremental) internal standard method, external standard method.
1. The advantage of the area normalization method is simple and accurate. When the operating conditions change, the effect on the results is small. It is suitable to analyze the content of each component in the multi-component sample. However, all components in the sample must be peaked. Therefore, this method is limited in use.
2. The external standard method (gas chromatography vials)is to use a pure substance to form a series of standard solutions of different concentrations (or directly purchase different concentration standard solutions) to take a certain volume, inject into the chromatograph, and make a standard curve according to the peak area and concentration. When analyzing the unknown, the concentration of the desired component is determined from the standard curve according to the same operating conditions and methods as the standard curve (the concentration can now be determined directly on the workstation). This method requires accurate injection, stable operating conditions, and the analytical sample and standard curve conditions must be consistent.
3. The internal standard method is applicable when all the components in the sample cannot be peaked or only one or a few components in the sample are required to be determined. The internal standard method is to accurately weigh a certain amount of sample, add a certain standard substance (internal standard), calculate the component to be tested according to the quality of the internal standard and the sample and the corresponding peak area on the chromatogram. content. The key to the internal standard method is to select the appropriate internal standard (gas chromatography vials). The internal standard should be a pure substance that is not present in the sample. The substance is similar to the substance to be tested and can be dissolved in the sample, but it cannot react with the sample. This method is more laborious and is generally not used for rapid analysis.
This is the end of the introduction of How to establish a gas chromatographic analysis method. I hope it can help you.
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