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1. Analysis of the difference between objects
GC: suitable for gasification, good thermal stability, and boiling point
Low sample; but for high boiling point, poor volatility,
Poor thermal stability, ionic and high polymer
Products, especially for most biochemical samples
20% of organic matter
HPLC: a sample that is suitable for solution after dissolution (including
Organic medium solution), free from sample volatility and
Limitation of thermal stability, large and difficult molecular weight
Biochemical samples with high gasification and poor thermal stability and high scores
Both sub- and ionic samples can be detected
Widely used, accounting for 80% of organic matter
2. Difference in mobile phase difference
GC: The mobile phase is inert, and the gas component has no affinity with the mobile phase.
Force, only interacts with the stationary phase.
HPLC: mobile phase is liquid, mobile phase and components have affinity
Force, can improve column selectivity, improve resolution, and separate
Positive action. And there are many types of mobile phases, and the choice is wide, and the change
Changing mobile phase polarity and pH also regulates separation.
Use two or more liquids in different proportions as the mobile phase
It is also possible to increase the separation selectivity.
3. Difference in operating conditions
GC: heating operation
HPLC: room temperature; high pressure (large viscosity of liquid, small peak broadening)
This is the end of the introduction of The difference between liquid chromatograph HPLC and gas chromatograph GC. I hope it can help you.
