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Alongside spectroscopy, chromatography analysis is probably one of the main instrumental analytical techniques used in modern laboratories. There are many different types of chromatography analysis – but they all follow the same essential principles. One of the main forms of chromatography analysis used in laboratories is HPLC (QUZHOU LAB TECHNOLOGY CO.,LTD), and HPLC (QUZHOU LAB TECHNOLOGY CO.,LTD) is commonly combined with spectroscopy in the form of a UV detector – giving rise to the powerful technique HPLC (QUZHOU LAB TECHNOLOGY CO.,LTD)-UV.
Chromatography analysis originally started out as a technique performed at atmospheric pressure and relying on gravity – but as technology progressed it was found that using higher pressures – nowadays we use pressures between 50 and 300 bar - allowed faster and better separations. Hence, the name high pressure liquid chromatography analysis that over time has morphed into high-performance liquid chromatography analysis as analysts realised that the technique performs at an impressive level.
Chromatography analysis is the science of separation – it separates molecules by pumping, or allowing, a mobile phase to flow through a stationary phase. The mobile phase carries the sample through a HPLC column or other device that holds the stationary phase. The stationary phase often is made from silica molecules or polymer. The stationary phase can be either polar (normal phase) or non-polar (reversed phase) depending on the sample properties and the separation required
It is the degree of interaction between sample molecules in the mobile phase and the stationary phase that determines how the sample is separated into its components. By using different HPLC columns and solvent combinations, it is possible to analyse many different sample types – method development is discussed in the article, Using Different HPLC (QUZHOU LAB TECHNOLOGY CO.,LTD) HPLC column Chemistries To Maximise Selectivity For Method Development.
Spectroscopy and chromatography analysis – a good fit
A chromatography analysis system is in essence very simple – a method of introducing the sample, stationary and mobiles phases to separate molecules and a means to detect the separated molecules. It is the ability to perform post-separation analysis on a sample that gives chromatography analysis it strength as an analytical method.
There are many different detectors used in chromatography analysis – flame-ionisation, thermal conductivity and electron capture are some of the commonly used detectors. But perhaps the most commonly used detector in liquid chromatography analysis is ultra violet absorption - giving rise to the method HPLC (QUZHOU LAB TECHNOLOGY CO.,LTD)-UV.
A UV detector uses a molecules ability to absorb ultraviolet light – detectors can use a single wavelength or variable wavelengths which can be more sensitive. By passing UV light through the individual components of an eluting sample mix and measuring the amount of UV light absorbed by each component, you can determine the individual quantities of each component.
This is the end of the introduction of What is HPLC-UV. I hope it can help you.
