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The Russian botanist Tswet discovered "chromatography" in 1903, and Martin and Synge proposed Liquid-Liquid Partition Chromatography in 1940, and in 1941 proposed the use of gas instead of liquid as the mobile phase. The possibility, 11 years later, James and Martin published a gas-Liquid Chromatography method from theory to practice, and thus won the Nobel Prize in Chemistry in 1952. On this basis, in 1957, MJEGolay pioneered Open-Tubular Column Chromatography, which is customarily called Capillary Column Chromatography. High-pressure pump and chemically bonded stationary phase for liquid chromatography, high-performance liquid chromatography (HPLC) 2ml chromatography vials, capillary supercritical fluid chromatography (SFC) developed in the early 1980s: and in the early 1980s by JORGENSON et al. The development of capillary electrophoresis (CZE) was widely developed and applied in the 1990s; capillary electrochromatography, which combines the advantages of HPLC and CZE, was valued in the late 1990s. [To the 21st century, chromatographic science will be at the forefront of life sciences. The field plays an irreplaceable role, and G.Guiochon believes that GC and HPLC are the best and most successful examples in the field of analysis.
In the field of modern analytical chemistry, chromatographic analysis is a novel separation analysis method. Chromatography can be used to separate and analyze complex multi-component mixtures, a method of separation based on the different migration velocities of the molecules. Chromatography is a separation process based on adsorption, partitioning, ion exchange, affinity and molecular size. It utilizes the adsorption capacity, partition coefficient, ion exchange capacity, affinity or molecular size of different components in two phases. Small differences, after several consecutive mass exchanges between the two phases, separate the different components. Chromatographic separation is a comprehensive representation of the thermodynamic and kinetic processes of a chromatographic system. A thermodynamic process is a process that is related to the partition coefficient of a component in a system; a kinetic process is a process in which components diffuse and mass transfer between two phases of the system 2ml chromatography vials. The thermodynamic properties of the components, mobile phase and stationary phase make the different components have different partition coefficients in the mobile phase and the stationary phase. The size of the partition coefficient reflects the dissolution-volatilization or adsorption-desorption of the components on the stationary phase. Ability. The component with a large partition coefficient has a strong dissolving or adsorbing ability on the stationary phase, so the moving speed in the column is slow; the component having a small partition coefficient dissolves or has a weak adsorption ability on the stationary phase, so the moving speed in the column is fast. After a certain period of time, due to the difference of the distribution coefficients, the components are differentially moved in the column to achieve the purpose of separation.
This is the end of the introduction of Principle of gas chromatography analysis. I hope it can help you.
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