Positive and negative chromatography

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Normal phase chromatography 2ml chromatography vials

Use a polar stationary phase (such as polyethylene glycol, amino and nitrile-bonded phases); the mobile phase is a relatively non-polar hydrophobic solvent (alkanes such as n-hexane, cyclohexane), often added to ethanol, different Propanol, tetrahydrofuran, chloroform, etc. to adjust the retention time of the components. It is commonly used to separate compounds with moderate polarity and polarity (such as phenols, amines, carbonyls and amino acids).

Reversed phase chromatography

2ml chromatography vials

Generally, a non-polar stationary phase (such as C18, C8) is used; the mobile phase is water or a buffer, and a water-miscible organic solvent such as methanol, acetonitrile, isopropanol, acetone or tetrahydrofuran is often added to adjust the retention time. Suitable for separating non-polar and weakly polar compounds. RPC is the most widely used in modern liquid chromatography, and it accounts for about 80% of the total HPLC application.2ml chromatography vials

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