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The HPLC vials has a depth of needle insertion. Also don't mistake the sample tray and HPLC vials.
That's it, the vials of the mobile phase, there is a very advanced operation, such as there will be in Agilent. That is, it has a prompt to automatically stop the pump when the mobile phase is less than a few percent. For example, less than 20%, people themselves have no mobile phase by default, and then stop. So after you have configured the mobile phase every day, you have to enter how many milliliters of mobile phase you have configured into your workstation. So it knows that there is still a mobile phase of 0.8L, and how much time is expected to go. Otherwise, after booting, it will continue to calculate yesterday's mobile phase, and then the mobile phase is still full, it thinks it is gone.
This is the end of the introduction of It shows nothing but actually there are still much liquid in the HPLC vials. I hope it can help you.
